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fluo-loaded hpasmcs  (Evident Corporation)

 
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    Structured Review

    Evident Corporation fluo-loaded hpasmcs
    circNFXL1 reversed the hypoxia-induced alterations in membrane potential and Kv currents in human pulmonary arterial smooth muscle cells <t>(hPASMCs).</t> A, circNFXL1 partially reversed the hypoxia-induced depolarization of resting membrane potential ( E m) in hPASMCs. The representative traces (B) and current-voltage relationships (IV) (C) of whole-cell Kv currents were recorded from hPASMCs in normoxia, hypoxia, and hypoxia+circNFXL1 conditions. D, The average amplitude of Kv currents recorded at +100 mV was compared between the 3 groups. * P < 0.05, ** P < 0.01, *** P < 0.001 (n = 10–12 cells). Mean ± SEM. Comparisons of data were acquired by one-way ANOVA followed by Bonferroni post-hoc. ANOVA, analysis of variance.
    Fluo Loaded Hpasmcs, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluo-loaded+hpasmcs/pmc10079296-81-14-15?v=Evident+Corporation
    Average 90 stars, based on 1 article reviews
    fluo-loaded hpasmcs - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "circNFXL1 Modulates the Kv2.1 Channel Function in Hypoxic Human Pulmonary Artery Smooth Muscle Cells via Sponging miR-29b-2-5p as a Competitive Endogenous RNA"

    Article Title: circNFXL1 Modulates the Kv2.1 Channel Function in Hypoxic Human Pulmonary Artery Smooth Muscle Cells via Sponging miR-29b-2-5p as a Competitive Endogenous RNA

    Journal: Journal of Cardiovascular Pharmacology

    doi: 10.1097/FJC.0000000000001396

    circNFXL1 reversed the hypoxia-induced alterations in membrane potential and Kv currents in human pulmonary arterial smooth muscle cells (hPASMCs). A, circNFXL1 partially reversed the hypoxia-induced depolarization of resting membrane potential ( E m) in hPASMCs. The representative traces (B) and current-voltage relationships (IV) (C) of whole-cell Kv currents were recorded from hPASMCs in normoxia, hypoxia, and hypoxia+circNFXL1 conditions. D, The average amplitude of Kv currents recorded at +100 mV was compared between the 3 groups. * P < 0.05, ** P < 0.01, *** P < 0.001 (n = 10–12 cells). Mean ± SEM. Comparisons of data were acquired by one-way ANOVA followed by Bonferroni post-hoc. ANOVA, analysis of variance.
    Figure Legend Snippet: circNFXL1 reversed the hypoxia-induced alterations in membrane potential and Kv currents in human pulmonary arterial smooth muscle cells (hPASMCs). A, circNFXL1 partially reversed the hypoxia-induced depolarization of resting membrane potential ( E m) in hPASMCs. The representative traces (B) and current-voltage relationships (IV) (C) of whole-cell Kv currents were recorded from hPASMCs in normoxia, hypoxia, and hypoxia+circNFXL1 conditions. D, The average amplitude of Kv currents recorded at +100 mV was compared between the 3 groups. * P < 0.05, ** P < 0.01, *** P < 0.001 (n = 10–12 cells). Mean ± SEM. Comparisons of data were acquired by one-way ANOVA followed by Bonferroni post-hoc. ANOVA, analysis of variance.

    Techniques Used:

    circNFXL1-regulated Kv2.1 expression via miR-29b-2 under hypoxic conditions. A, qPCR and (B) WB analysis of Kv2.1 in hPASMCs transfected with circNFXL1 expression vector or circNFXL1+miR-29b-2 mix and cultured under hypoxic conditions for 24–48 hours (hypoxia/circNFXL1, hypoxia/circNFXL1 + miR29b). hPASMCs transfected with pLC5-ciR and cultured under normal/hypoxic conditions were used as controls (normoxia/hypoxia). ** P < 0.01(n = 5 individual experiments). Mean ± SEM. Comparisons of data were acquired by one-way ANOVA followed by Bonferroni post-hoc. ANOVA, analysis of variance.
    Figure Legend Snippet: circNFXL1-regulated Kv2.1 expression via miR-29b-2 under hypoxic conditions. A, qPCR and (B) WB analysis of Kv2.1 in hPASMCs transfected with circNFXL1 expression vector or circNFXL1+miR-29b-2 mix and cultured under hypoxic conditions for 24–48 hours (hypoxia/circNFXL1, hypoxia/circNFXL1 + miR29b). hPASMCs transfected with pLC5-ciR and cultured under normal/hypoxic conditions were used as controls (normoxia/hypoxia). ** P < 0.01(n = 5 individual experiments). Mean ± SEM. Comparisons of data were acquired by one-way ANOVA followed by Bonferroni post-hoc. ANOVA, analysis of variance.

    Techniques Used: Expressing, Transfection, Plasmid Preparation, Cell Culture

    circNFXL1-regulated intracellular calcium via sponging miR-29b-2 under hypoxic conditions. Intracellular calcium level was measured using the Fluo-3-AM staining. The hPASMCs transfected with circNFXL1 expression vector or circNFXL1+miR-29b-2 mix and cultured under hypoxic conditions for 24–48 hours (hypoxia/circNFXL1, hypoxia/circNFXL1+miR29b). hPASMCs transfected with pLC5-ciR and cultured under normal/hypoxic conditions were used as controls (normoxia/hypoxia). After transfections, the cultured hPASMCs were labeled by Fura-3-AM. Detection of intracellular Ca 2+ was carried out by imaging the fluorescence intensity of fluo-loaded hPASMCs. * P < 0.05, ** P < 0.01 (n = 8–10 cells). Mean ± SEM. Comparisons of data were acquired by one-way ANOVA followed by Bonferroni post-hoc. ANOVA, analysis of variance.
    Figure Legend Snippet: circNFXL1-regulated intracellular calcium via sponging miR-29b-2 under hypoxic conditions. Intracellular calcium level was measured using the Fluo-3-AM staining. The hPASMCs transfected with circNFXL1 expression vector or circNFXL1+miR-29b-2 mix and cultured under hypoxic conditions for 24–48 hours (hypoxia/circNFXL1, hypoxia/circNFXL1+miR29b). hPASMCs transfected with pLC5-ciR and cultured under normal/hypoxic conditions were used as controls (normoxia/hypoxia). After transfections, the cultured hPASMCs were labeled by Fura-3-AM. Detection of intracellular Ca 2+ was carried out by imaging the fluorescence intensity of fluo-loaded hPASMCs. * P < 0.05, ** P < 0.01 (n = 8–10 cells). Mean ± SEM. Comparisons of data were acquired by one-way ANOVA followed by Bonferroni post-hoc. ANOVA, analysis of variance.

    Techniques Used: Staining, Transfection, Expressing, Plasmid Preparation, Cell Culture, Labeling, Imaging, Fluorescence



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    fluo-loaded hpasmcs  (Evident Corporation)
    90
    Evident Corporation fluo-loaded hpasmcs
    circNFXL1 reversed the hypoxia-induced alterations in membrane potential and Kv currents in human pulmonary arterial smooth muscle cells <t>(hPASMCs).</t> A, circNFXL1 partially reversed the hypoxia-induced depolarization of resting membrane potential ( E m) in hPASMCs. The representative traces (B) and current-voltage relationships (IV) (C) of whole-cell Kv currents were recorded from hPASMCs in normoxia, hypoxia, and hypoxia+circNFXL1 conditions. D, The average amplitude of Kv currents recorded at +100 mV was compared between the 3 groups. * P < 0.05, ** P < 0.01, *** P < 0.001 (n = 10–12 cells). Mean ± SEM. Comparisons of data were acquired by one-way ANOVA followed by Bonferroni post-hoc. ANOVA, analysis of variance.
    Fluo Loaded Hpasmcs, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluo-loaded+hpasmcs/pmc10079296-81-14-15?v=Evident+Corporation
    Average 90 stars, based on 1 article reviews
    fluo-loaded hpasmcs - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    circNFXL1 reversed the hypoxia-induced alterations in membrane potential and Kv currents in human pulmonary arterial smooth muscle cells (hPASMCs). A, circNFXL1 partially reversed the hypoxia-induced depolarization of resting membrane potential ( E m) in hPASMCs. The representative traces (B) and current-voltage relationships (IV) (C) of whole-cell Kv currents were recorded from hPASMCs in normoxia, hypoxia, and hypoxia+circNFXL1 conditions. D, The average amplitude of Kv currents recorded at +100 mV was compared between the 3 groups. * P < 0.05, ** P < 0.01, *** P < 0.001 (n = 10–12 cells). Mean ± SEM. Comparisons of data were acquired by one-way ANOVA followed by Bonferroni post-hoc. ANOVA, analysis of variance.

    Journal: Journal of Cardiovascular Pharmacology

    Article Title: circNFXL1 Modulates the Kv2.1 Channel Function in Hypoxic Human Pulmonary Artery Smooth Muscle Cells via Sponging miR-29b-2-5p as a Competitive Endogenous RNA

    doi: 10.1097/FJC.0000000000001396

    Figure Lengend Snippet: circNFXL1 reversed the hypoxia-induced alterations in membrane potential and Kv currents in human pulmonary arterial smooth muscle cells (hPASMCs). A, circNFXL1 partially reversed the hypoxia-induced depolarization of resting membrane potential ( E m) in hPASMCs. The representative traces (B) and current-voltage relationships (IV) (C) of whole-cell Kv currents were recorded from hPASMCs in normoxia, hypoxia, and hypoxia+circNFXL1 conditions. D, The average amplitude of Kv currents recorded at +100 mV was compared between the 3 groups. * P < 0.05, ** P < 0.01, *** P < 0.001 (n = 10–12 cells). Mean ± SEM. Comparisons of data were acquired by one-way ANOVA followed by Bonferroni post-hoc. ANOVA, analysis of variance.

    Article Snippet: Detection of intracellular Ca 2+ was conducted by imaging the fluorescence intensity of fluo-loaded hPASMCs (OLYMPUS, BX51).

    Techniques:

    circNFXL1-regulated Kv2.1 expression via miR-29b-2 under hypoxic conditions. A, qPCR and (B) WB analysis of Kv2.1 in hPASMCs transfected with circNFXL1 expression vector or circNFXL1+miR-29b-2 mix and cultured under hypoxic conditions for 24–48 hours (hypoxia/circNFXL1, hypoxia/circNFXL1 + miR29b). hPASMCs transfected with pLC5-ciR and cultured under normal/hypoxic conditions were used as controls (normoxia/hypoxia). ** P < 0.01(n = 5 individual experiments). Mean ± SEM. Comparisons of data were acquired by one-way ANOVA followed by Bonferroni post-hoc. ANOVA, analysis of variance.

    Journal: Journal of Cardiovascular Pharmacology

    Article Title: circNFXL1 Modulates the Kv2.1 Channel Function in Hypoxic Human Pulmonary Artery Smooth Muscle Cells via Sponging miR-29b-2-5p as a Competitive Endogenous RNA

    doi: 10.1097/FJC.0000000000001396

    Figure Lengend Snippet: circNFXL1-regulated Kv2.1 expression via miR-29b-2 under hypoxic conditions. A, qPCR and (B) WB analysis of Kv2.1 in hPASMCs transfected with circNFXL1 expression vector or circNFXL1+miR-29b-2 mix and cultured under hypoxic conditions for 24–48 hours (hypoxia/circNFXL1, hypoxia/circNFXL1 + miR29b). hPASMCs transfected with pLC5-ciR and cultured under normal/hypoxic conditions were used as controls (normoxia/hypoxia). ** P < 0.01(n = 5 individual experiments). Mean ± SEM. Comparisons of data were acquired by one-way ANOVA followed by Bonferroni post-hoc. ANOVA, analysis of variance.

    Article Snippet: Detection of intracellular Ca 2+ was conducted by imaging the fluorescence intensity of fluo-loaded hPASMCs (OLYMPUS, BX51).

    Techniques: Expressing, Transfection, Plasmid Preparation, Cell Culture

    circNFXL1-regulated intracellular calcium via sponging miR-29b-2 under hypoxic conditions. Intracellular calcium level was measured using the Fluo-3-AM staining. The hPASMCs transfected with circNFXL1 expression vector or circNFXL1+miR-29b-2 mix and cultured under hypoxic conditions for 24–48 hours (hypoxia/circNFXL1, hypoxia/circNFXL1+miR29b). hPASMCs transfected with pLC5-ciR and cultured under normal/hypoxic conditions were used as controls (normoxia/hypoxia). After transfections, the cultured hPASMCs were labeled by Fura-3-AM. Detection of intracellular Ca 2+ was carried out by imaging the fluorescence intensity of fluo-loaded hPASMCs. * P < 0.05, ** P < 0.01 (n = 8–10 cells). Mean ± SEM. Comparisons of data were acquired by one-way ANOVA followed by Bonferroni post-hoc. ANOVA, analysis of variance.

    Journal: Journal of Cardiovascular Pharmacology

    Article Title: circNFXL1 Modulates the Kv2.1 Channel Function in Hypoxic Human Pulmonary Artery Smooth Muscle Cells via Sponging miR-29b-2-5p as a Competitive Endogenous RNA

    doi: 10.1097/FJC.0000000000001396

    Figure Lengend Snippet: circNFXL1-regulated intracellular calcium via sponging miR-29b-2 under hypoxic conditions. Intracellular calcium level was measured using the Fluo-3-AM staining. The hPASMCs transfected with circNFXL1 expression vector or circNFXL1+miR-29b-2 mix and cultured under hypoxic conditions for 24–48 hours (hypoxia/circNFXL1, hypoxia/circNFXL1+miR29b). hPASMCs transfected with pLC5-ciR and cultured under normal/hypoxic conditions were used as controls (normoxia/hypoxia). After transfections, the cultured hPASMCs were labeled by Fura-3-AM. Detection of intracellular Ca 2+ was carried out by imaging the fluorescence intensity of fluo-loaded hPASMCs. * P < 0.05, ** P < 0.01 (n = 8–10 cells). Mean ± SEM. Comparisons of data were acquired by one-way ANOVA followed by Bonferroni post-hoc. ANOVA, analysis of variance.

    Article Snippet: Detection of intracellular Ca 2+ was conducted by imaging the fluorescence intensity of fluo-loaded hPASMCs (OLYMPUS, BX51).

    Techniques: Staining, Transfection, Expressing, Plasmid Preparation, Cell Culture, Labeling, Imaging, Fluorescence